PHYLOGENOMIC, STRUCTURAL, AND CELL BIOLOGICAL ANALYSES REVEAL THAT STENOTROPHOMONAS MALTOPHILIA REPLICATES IN ACIDIFIED RAB7A-POSITIVE VACUOLES OF ACANTHAMOEBA CASTELLANII

ABSTRACTAcanthamoeba species are clinically relevant free-living amoebae (FLA) ubiquitously found in soil and water bodies.Metabolically active trophozoites graze on diverse microbes via phagocytosis.However, functional studies on Rab GTPases (Rabs), which are critical for controlling vesicle trafficking and maturation, are scarce for this Baggers

(1)Objective: In this study, a quantitative analysis of chemical groups (the triterpenoids, water-soluble polysaccharides, and acidic polysaccharides) and quantitative high liquid performance chromatography (HPLC) fingerprint of Poria cocos (Schw.) Wolf (PC) for quality control was developed.(2) Light Meters Methodology: First, three main chemical